A pilot field experiment to assess the relationship between traditional biogeochemical rate measurements and transcriptional activity of microbial populations was carried out at the LEO 15 site off Tuckerton, N. Similar diel patterns of carbon fixation and rbcL gene expression were observed in three of four time series, with maxima for photosynthetic capacity P max and rbcL mRNA occurring between 10 a. The lowest P max and rbcL levels were detected between 6 p. Clone libraries from reverse transcription-PCR-amplified rbcL mRNA indicated the presence of several chromophytic algae diatoms, prymnesiophytes, and chrysophytes and some eukaryotic green flagellates. Analogous results were obtained from amplified small rRNA sequences and secondary pigment analysis. These results suggest that diatoms were a major contributor to carbon fixation at LEO 15 at the time of sampling and that photosynthetic carbon fixation was partially controlled by transcriptional regulation of the RubisCO gene. A primary research goal for marine environmental biogeochemistry is a thorough understanding of the molecular to global scale links and feedback mechanisms between solar irradiance, marine microbial activity, primary productivity, carbon and nitrogen cycles, and remotely sensed ocean color data. Increased emphasis is now being placed on the application of modern molecular tools to address these problems. Understanding of the genetic basis of biogeochemical processes and, more importantly, the expression of relevant genes in the marine environment is perceived as a promising route to these goals. A detailed understanding of the connection between gene expression, protein translation, and enzyme activity in environmental systems can provide significant insight into the mechanisms controlling biogeochemical transformation rates.
MATERIALS AND METHODS
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View research View latest news Sign up for updates. Both organisms were grown on a light-dark cycle, with the light period starting at Strong diel patterns in these three aspects of carbon fixation occurred in both organisms, with maximal levels in the light period and minima in the dark. In Synechococcus, maximal rbcL transcript abundance occurred at noon and was followed by rapid disappearance. RubisCO enzyme activity and whole cell carbon fixation were elevated at , and they disappeared over the next 8 hours. A similar diel pattern of cyanobacterial-like and chromophyte-like rbcL transcription has been observed in natural phytoplankton populations. These studies suggest that chromophytes are more adapted to take advantage of carbon fixation late in the day than cyanophytes. This is a preview of subscription content, log in to check access. Rent this article via DeepDyve. Reprints and Permissions.
DNase I footprint analyses indicated that R. A cosmid carrying the R. DNase I footprint analyses, using R. Expression from promoter insertion mutants showed that the function of the promoter distal regulatory region was helical phase-dependent. These results indicated that RegA exerts its regulatory affect on cbb I expression through direct interaction with the cbb I promoter. The costs of publication of this article were defrayed in part by the payment of page charges. Section solely to indicate this fact. You'll be in good company. Journal of Lipid Research.